Herbert D C, Sheridan P J
Biol Reprod. 1984 Mar;30(2):479-83. doi: 10.1095/biolreprod30.2.479.
The identification of target cells for progesterone was conducted on pituitary glands obtained from long-term ovariectomized and adrenalectomized female baboons that had been injected with 3H-ORG 2058 (16 alpha-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione; Amersham Corp). Binding sites were localized using autoradiography alone or in conjunction with immunocytochemistry. The synthetic progestin was primarily found over nuclei of cells in the pars distalis and pars intermedia, although a small population of pituicytes also bound the 3H-ORG 2058. The majority of steroid-sensitive cells in the pars distalis were gonadotrophs. These observations suggest a role for progesterone in the regulation of not only the gonadotropins but also those hormones secreted by the cells of the pars intermedia.
对从长期卵巢切除和肾上腺切除的雌性狒狒获得的垂体进行孕酮靶细胞鉴定，这些狒狒已注射3H-ORG 2058（16α-乙基-21-羟基-19-去甲-4-孕烯-3,20-二酮；阿默沙姆公司）。使用放射自显影单独或与免疫细胞化学结合来定位结合位点。合成孕激素主要在远侧部和中间部细胞的细胞核上发现，尽管一小部分垂体细胞也结合3H-ORG 2058。远侧部中对类固醇敏感的大多数细胞是促性腺激素细胞。这些观察结果表明孕酮不仅在促性腺激素的调节中起作用，而且在中间部细胞分泌的那些激素的调节中也起作用。
