Wenger G D, Balcerzak S P
Blood. 1984 Apr;63(4):897-903.
The rates of polymerization and depolymerization of identical concentrated deoxygenated hemoglobin S (HbS) solutions following a rapid temperature change were examined by several methods. Two of these methods measured viscosity changes in either gently agitated (AGT) or nonagitated (NAGT) samples. The third method utilized a change in turbidity at 735 nm (SDT). By all three methods, a delay period, during which no observable change was detected, followed the temperature change. Gelation, as determined in nonagitated samples by a viscosity-based technique, occurred before or coincided with gelation as determined spectrophotometrically. The slope of the concentration dependence of the delay time is significantly decreased by agitation. Similar monitoring of the depolymerization reaction indicated the persistence of increased viscosity after observation of a marked decrease in turbidity.
通过几种方法研究了相同浓度的脱氧血红蛋白 S（HbS）溶液在快速温度变化后的聚合和解聚速率。其中两种方法测量了轻轻搅拌（AGT）或未搅拌（NAGT）样品中的粘度变化。第三种方法利用了 735 nm 处的浊度变化（SDT）。通过所有这三种方法，在温度变化之后都有一个延迟期，在此期间未检测到可观察到的变化。通过基于粘度的技术在未搅拌样品中确定的凝胶化，在通过分光光度法确定的凝胶化之前发生或与之同时发生。搅拌可显著降低延迟时间的浓度依赖性斜率。对解聚反应的类似监测表明，在观察到浊度显著降低后，粘度仍持续升高。
