Goon D, Conner M K
Carcinogenesis. 1984 Mar;5(3):399-402. doi: 10.1093/carcin/5.3.399.
At 3, 6, and 24 h after acute treatment of BD2F1 mice with 3.3 mmol/kg of ethyl carbamate, blood was drawn and cultured for SCE analysis. After blood sampling, the same mice were infused with BrdUrd for simultaneous assessment of SCEs in bone marrow and alveolar macrophage cells. In bone marrow and alveolar macrophage cells, maximum SCE responses (24.1 +/- 4.3; 24.2 +/- 3.8, respectively) were observed at 3 h post-exposure. Decreased SCE frequencies were observed at each later time. Relative to a single (3.3 mmol/kg) acute treatment, multiple treatment (10 X 3.3 mmol/kg; every other day) did not produce significantly elevated SCE levels in bone marrow (18.3 +/- 1.5) and alveolar macrophage cells (22.2 +/- 5.8). By contrast, the maximum lymphocyte response (18.8 +/- 2.2), following a single acute injection, was observed at 6 h post-exposure. In addition, a considerable accumulation of SCE frequencies occurred in lymphocytes (27.3 +/- 3.4) following multiple injections of ethyl carbamate.
用3.3 mmol/kg氨基甲酸乙酯对BD2F1小鼠进行急性处理后3小时、6小时和24小时，采集血液并培养用于姐妹染色单体交换（SCE）分析。采血后，向同一批小鼠注射溴脱氧尿苷（BrdUrd），以便同时评估骨髓和肺泡巨噬细胞中的SCE。在骨髓和肺泡巨噬细胞中，暴露后3小时观察到最大SCE反应（分别为24.1±4.3；24.2±3.8）。在随后的每个时间点，SCE频率均降低。相对于单次（3.3 mmol/kg）急性处理，多次处理（10×3.3 mmol/kg；每隔一天一次）并未使骨髓（18.3±1.5）和肺泡巨噬细胞（22.2±5.8）中的SCE水平显著升高。相比之下，单次急性注射后，在暴露后6小时观察到最大淋巴细胞反应（18.8±2.2）。此外，多次注射氨基甲酸乙酯后，淋巴细胞中的SCE频率出现了相当大的累积（27.3±3.4）。
