Aidoo A, Morris S M, Domon O E, McGarrity L J, Kodell R L, Casciano D A
Division of Genetic Toxicology, National Center for Toxicological Research, Jefferson, Arkansas 72079.
Cell Biol Toxicol. 1989 Nov;5(3):237-48. doi: 10.1007/BF01795353.
2-Mercaptoethanol (2-ME) is used as a medium supplement to enhance the proliferation of lymphocytes cultured in vitro. In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocytes. There were virtually no metaphases detected in cells cultured without PHA. In PHA-stimulated cultures, 2-ME decreased SCE frequency but it enhanced SCE frequency in the presence of 5 to 12.5 microM bromodeoxyuridine (BRdU). Both mitotic and replication indices were increased in the PHA/2-ME system. The levels of incorporated exogenous thymidine, in the presence of 2-ME, were relatively low in unstimulated cells, suggesting that 2-ME is not mitogenic for T-cells. However, 2-ME enhanced PHA-induced response of T-cells as evidenced by increased levels of thymidine incorporation into cellular DNA. The growth-promoting effects and the decrease in SCE frequency caused by 2-ME upon PHA stimulation indicate that 2-ME may alter the nature of interaction between PHA and cellular activating properties or the replicative processes.
2-巯基乙醇（2-ME）用作培养基添加剂，以增强体外培养的淋巴细胞的增殖。在本研究中，我们检测了2-ME对未刺激的和经植物血凝素（PHA）刺激的Fischer 344大鼠淋巴细胞培养物中细胞生长和姐妹染色单体交换（SCE）诱导的影响。在无PHA培养的细胞中几乎检测不到中期分裂相。在PHA刺激的培养物中，2-ME降低了SCE频率，但在存在5至12.5微摩尔溴脱氧尿苷（BRdU）的情况下它提高了SCE频率。PHA/2-ME系统中的有丝分裂指数和复制指数均增加。在存在2-ME的情况下，未刺激细胞中外源胸腺嘧啶核苷的掺入水平相对较低，这表明2-ME对T细胞无促有丝分裂作用。然而，2-ME增强了PHA诱导的T细胞反应，细胞DNA中胸腺嘧啶核苷掺入水平的增加证明了这一点。2-ME在PHA刺激时的生长促进作用和SCE频率降低表明，2-ME可能改变PHA与细胞活化特性之间相互作用的性质或复制过程。
