Pasquali-Ronchetti I, Bressan G M, Fornieri C, Baccarani-Contri M, Castellani I, Volpin D
Exp Mol Pathol. 1984 Apr;40(2):235-45. doi: 10.1016/0014-4800(84)90080-7.
Ruthenium red and toluidine blue O precipitates were described associated with lathyritic elastic fibers in aortas of chickens treated with beta-aminopropionitrile fumarate (I. Pasquali-Ronchetti, C. Fornieri, I. Castellani, G. M. Bressan, and D. Volpin (1981). Alterations of the connective tissue components induced by beta-aminopropionitrile. Exp. Mol. Pathol. 35, 42-56). In this report evidence is given that these precipitates reveal the presence of proteoglycans, as they are completely removed by 5 M guanidine-HCl incubation and by specific enzymatic digestions. In particular, proteoglycans associated with the poorly cross-linked lathyritic elastin can be removed by testicular hyaluronidase, chondroitinase ABC, heparitinase, and nitrous acid treatments, whereas they are rather resistant to streptococcal hyaluronidase and chondroitinase AC. On the contrary, proteoglycans of the matrix or associated with collagen fibers are particularly sensitive to these latter enzymatic treatments. The conclusion is reached that glycosaminoglycans associated with beta-aminopropionitrile-induced lathyritic elastin (i) are different from those of the matrix or associated with collagen, and (ii) include mainly dermatan and heparan sulfates.
在用富马酸β-氨基丙腈处理的鸡主动脉中，钌红和甲苯胺蓝O沉淀与致跛弹性纤维有关（I. 帕斯夸利 - 龙凯蒂、C. 福尔涅里、I. 卡斯特拉尼、G. M. 布雷桑和D. 沃尔平（1981年）。β-氨基丙腈诱导的结缔组织成分改变。实验与分子病理学。35，42 - 56）。在本报告中，有证据表明这些沉淀揭示了蛋白聚糖的存在，因为它们通过5M盐酸胍孵育和特定的酶消化可被完全去除。特别是，与交联不良的致跛弹性蛋白相关的蛋白聚糖可通过睾丸透明质酸酶、软骨素酶ABC、肝素酶和亚硝酸处理去除，而它们对链球菌透明质酸酶和软骨素酶AC相当耐受。相反，基质中的蛋白聚糖或与胶原纤维相关的蛋白聚糖对后一种酶处理特别敏感。得出的结论是，与β-氨基丙腈诱导的致跛弹性蛋白相关的糖胺聚糖（i）与基质中的或与胶原相关的糖胺聚糖不同，且（ii）主要包括硫酸皮肤素和硫酸乙酰肝素。
