Do pup ultrasonic cries provoke prolactin secretion in lactating rats?

Marked prolactin (PRL) secretion in response to the ultrasonic distress vocalizations of rat pups in lactating dams deprived of their pups for 6 hr was reported by others. In two experiments, this phenomenon could not be confirmed under our testing conditions at either 1 or 2 weeks postpartum, although behavioral responses to the ultrasounds were noted. In addition, suckling-induced PRL secretion did not differ consistently as a function of the tape recording (pup ultrasounds, 45 kHz artificially produced ultrasounds, or blank tape) heard prior to the return of pups. The functional significance of rat pup ultrasounds is considered.