Dutt M K
Folia Histochem Cytochem (Krakow). 1976;14(4):309-14.
In the present study rat liver pieces fixed in 1) 10 per cent buffered neutral formalin, 2) 4 per cent glutaraldehyde, 3) Heidenhain's-Susa fixative and 4) Flemming's fluid, and following hydrolysis in 1-0 N HC1 at 60degreesC for varying time periods have been stained with the UV Feulgen procedure. The results of this study reveal that following hydrolysis for different time periods the tissue material fixed in formalin show the same staining pattern as those fixed in glutaraldehyde. The material fixed in Heidenhain's-Susa displays an intense Feulgen staining after two different times of hydrolysis, and that fixed in Flemming's fluid shows particular staining intensity for a prolonged time period thus indicating better preservation of DNA than in the materials fixed in the other three fixtatives.
在本研究中，用以下四种固定剂固定的大鼠肝脏切片，即1）10%缓冲中性福尔马林、2）4%戊二醛、3）海登海因氏-苏萨固定液和4）弗莱明氏液，在60℃下于1.0N盐酸中水解不同时间段后，采用紫外福尔根染色法进行染色。本研究结果表明，在不同时间段水解后，用福尔马林固定的组织材料与用戊二醛固定的组织材料呈现相同的染色模式。用海登海因氏-苏萨固定液固定的材料在两次不同的水解时间后显示出强烈的福尔根染色，而用弗莱明氏液固定的材料在较长时间段内显示出特定的染色强度。因此，这表明其DNA的保存比用其他三种固定剂固定的材料更好。
