Dutt M K
Folia Histochem Cytochem (Krakow). 1975;13(3-4):195-9.
In the experiments sections of rat tissues fixed in 10 per cent buffered neutral formalin for 3 hour and treated for 15 minutes with different chemical reagents such as pyridine, tributylamine, urea, tris sodium nitrite and sodium hydroxide were subjected to hydrolysis in 6 N HCl at 25 degrees C for 20 minutes and stained by the UV Feulgen technique. The results reveal a far more intense staining of the nuclei in tissues treated with any of the chemicals mentioned than in untreated controls. The possible role of these chemicals in enhancing the staining intensity is discussed.
在实验部分，将大鼠组织用10%缓冲中性福尔马林固定3小时，并用吡啶、三丁胺、尿素、亚硝酸三钠和氢氧化钠等不同化学试剂处理15分钟，然后在25℃下于6N盐酸中水解20分钟，并用紫外孚尔根技术染色。结果显示，用上述任何一种化学试剂处理的组织中的细胞核染色比未处理的对照组织强烈得多。讨论了这些化学试剂在增强染色强度方面可能的作用。
