Dutt M K
Folia Histochem Cytochem (Krakow). 1975;13(3-4):201-5.
Sections of rat liver fixed in CRAF III and Nawaschin's fixative in Dutt's modification were subjected to hydrolysis in 1N HCl at 60 degrees C for different periods of time and to Schiff's staining according to the UV Feulgen technique. The study showed that Feulgen reaction intensity depends upon time of hydrolysis, optimum coloration being possible only after 10-15 min of hydrolysis. Prolongation of hydrolysis beyond this time produced decreased staining intensity which is retained for further 35 min of hydrolysis thus forming a plateau. Further prolongation of hydrolysis results in gradual deterioration of the staining intensity which culminates in utterly pale coloration of the nuclei after one hour's hydrolysis. A possible explanation for this phenomena is suggested.
将用杜特改良的CRAF III和纳瓦申固定液固定的大鼠肝脏切片，在60℃的1N盐酸中水解不同时间，然后按照紫外福尔根技术进行席夫氏染色。研究表明，福尔根反应强度取决于水解时间，只有在水解10 - 15分钟后才可能出现最佳染色效果。水解时间超过此范围会导致染色强度降低，这种降低在进一步水解35分钟时保持不变，从而形成一个平稳期。水解时间进一步延长会导致染色强度逐渐下降，在水解一小时后细胞核最终完全褪色。文中提出了对此现象的一种可能解释。
