头孢哌酮对乙醇和乙醛体外代谢的影响。

Influence of cefoperazone on ethanol and acetaldehyde metabolism in vitro.

作者信息

Greizerstein H B, Siemens A J

出版信息

J Antibiot (Tokyo). 1984 Feb;37(2):167-71. doi: 10.7164/antibiotics.37.167.

DOI:10.7164/antibiotics.37.167

Abstract

The effect of cefoperazone on ethanol and acetaldehyde metabolism was studied in rat liver homogenates and with a purified aldehyde dehydrogenase. Rat liver homogenates were incubated with ethanol (30 mM) alone or in combination with cefoperazone (15 or 150 micrograms/g liver). Ethanol and acetaldehyde concentrations were determined at 6, 12, 18 and 24 minutes. Cefoperazone added to the incubation medium inhibited ethanol and acetaldehyde metabolism in a concentration-dependent manner. The addition of cefoperazone to rat liver homogenates incubated with acetaldehyde (300 microM), however, did not inhibit acetaldehyde disappearance for a period of 15 minutes. Purified aldehyde dehydrogenase was incubated with 300 microM acetaldehyde. When cefoperazone was added, acetaldehyde disappearance was significantly slower than without cefoperazone. The data indicate that cefoperazone inhibits ethanol metabolism in rat liver homogenates in a concentration-dependent manner. The effect of the antibiotic on acetaldehyde elimination in liver homogenate, however, depends on the concentration of acetaldehyde in the medium. The acetaldehyde dehydrogenase obtained from yeast is inhibited by cefoperazone.

摘要

在大鼠肝脏匀浆和纯化的醛脱氢酶中研究了头孢哌酮对乙醇和乙醛代谢的影响。大鼠肝脏匀浆单独与乙醇（30 mM）孵育，或与头孢哌酮（15或150微克/克肝脏）联合孵育。在6、12、18和24分钟时测定乙醇和乙醛浓度。添加到孵育培养基中的头孢哌酮以浓度依赖性方式抑制乙醇和乙醛代谢。然而，将头孢哌酮添加到与乙醛（300 microM）孵育的大鼠肝脏匀浆中，在15分钟内并未抑制乙醛的消失。纯化的醛脱氢酶与300 microM乙醛孵育。添加头孢哌酮时，乙醛消失明显慢于未添加头孢哌酮时。数据表明，头孢哌酮以浓度依赖性方式抑制大鼠肝脏匀浆中的乙醇代谢。然而，抗生素对肝脏匀浆中乙醛消除的影响取决于培养基中乙醛的浓度。从酵母中获得的乙醛脱氢酶受到头孢哌酮的抑制。