Flick D A, Gifford G E
J Immunol Methods. 1984 Mar 30;68(1-2):167-75. doi: 10.1016/0022-1759(84)90147-9.
Four published in vitro assays which measure cell cytotoxicity were compared utilizing murine tumor necrosis factor. These included determination of residual cell number by crystal violet staining in the presence and absence of actinomycin D, lack of viability as determined by neutral red uptake, and [3H]thymidine release in cytotoxin treated L929 cells. Treatment of cells with actinomycin D followed by crystal violet staining was the most sensitive method measured. However, addition of actinomycin D to the neutral red uptake assay could be shown to be even more sensitive. Additionally, it was shown how actinomycin D dosage, cell seeding density and time of incubation affect TNF titer.
利用鼠肿瘤坏死因子比较了四种已发表的测量细胞毒性的体外试验。这些试验包括在有和没有放线菌素D的情况下通过结晶紫染色测定残留细胞数、通过中性红摄取测定细胞活力丧失以及细胞毒素处理的L929细胞中的[3H]胸腺嘧啶核苷释放。用放线菌素D处理细胞后进行结晶紫染色是所测量的最敏感方法。然而，在中性红摄取试验中加入放线菌素D被证明更敏感。此外，还展示了放线菌素D剂量、细胞接种密度和孵育时间如何影响肿瘤坏死因子效价。
