Black E G, Sheppard M C, Hoffenberg R
J Endocrinol. 1984 Apr;101(1):107-11. doi: 10.1677/joe.0.1010107.
Serum thyroglobulin (Tg), measured by radioimmunoassay, was high in 6-propylthiouracil (PTU)-treated rats but low in thyroxine (T4)-treated animals compared with euthyroid controls. Thyroid-stimulating hormone (TSH) stimulated Tg release in vitro from enzymatically dispersed normal rat thyroid cells in a dose-dependent manner. Thyroid cells prepared from T4-treated animals behaved similarly to cells from control rats, whereas in vitro basal release of Tg from thyroid cells prepared from PTU-treated animals was high and the response to TSH was lost. Our data confirm the TSH dependency of Tg release in vivo and in vitro and our system provides a means of studying the control of Tg secretion in vitro.
通过放射免疫测定法测得，与甲状腺功能正常的对照动物相比，用6-丙基硫氧嘧啶（PTU）治疗的大鼠血清甲状腺球蛋白（Tg）含量较高，而用甲状腺素（T4）治疗的动物血清甲状腺球蛋白含量较低。促甲状腺激素（TSH）以剂量依赖的方式刺激体外酶分散的正常大鼠甲状腺细胞释放Tg。从T4治疗的动物制备的甲状腺细胞与对照大鼠的细胞表现相似，而从PTU治疗的动物制备的甲状腺细胞的体外基础Tg释放量很高，并且对TSH的反应丧失。我们的数据证实了体内和体外Tg释放对TSH的依赖性，并且我们的系统提供了一种体外研究Tg分泌控制的方法。
