Kawaoi A, Tsuneda M
Endocrinol Jpn. 1986 Dec;33(6):835-41. doi: 10.1507/endocrj1954.33.835.
In order to elucidate the maternal factors influencing the functional development of the fetal rat thyroid gland, pregnant rats were subjected to either thyroidectomy or administration of PTU or TSH and the thyroid glands of the fetuses were examined chronologically by immunohistochemistry to detect thyroglobulin (Tg), T4 and T3. In the group undergoing thyroidectomy, the occurrence of immunoreactive Tg, T4 and T3 was the same as in the control group in spite of slight retardation of the development of the thyroid gland. On the other hand, PTU administration caused remarkable degeneration of the hyperplastic epithelium of the follicles, where immunoreactivity of T4 and T3 was barely detectable, suggesting a transplacental effect of PTU on the fetal thyroid gland. However, Tg remained unaffected and was stained as well as in the controls. Injection of TSH led to a delay in the occurrence of T4 and T3 by one day, probably due to increased levels of thyroid hormone from the stimulated thyroid gland of the mother rats.
为了阐明影响胎鼠甲状腺功能发育的母体因素，对怀孕大鼠进行甲状腺切除术或给予丙硫氧嘧啶（PTU）或促甲状腺激素（TSH），并通过免疫组织化学按时间顺序检查胎儿的甲状腺，以检测甲状腺球蛋白（Tg）、T4和T3。在接受甲状腺切除术的组中，尽管甲状腺发育略有延迟，但免疫反应性Tg、T4和T3的出现情况与对照组相同。另一方面，给予PTU导致滤泡增生上皮显著退化，几乎检测不到T4和T3的免疫反应性，提示PTU对胎儿甲状腺有经胎盘效应。然而，Tg未受影响，染色情况与对照组一样。注射TSH导致T4和T3的出现延迟一天，这可能是由于母鼠受刺激的甲状腺产生的甲状腺激素水平升高所致。
