Yamamoto K, Johnston R B
J Exp Med. 1984 Feb 1;159(2):405-16. doi: 10.1084/jem.159.2.405.
We explored the relationship between phagocytosis and the triggering of oxidative metabolism using resident, lipopolysaccharide (LPS)-elicited, and bacille Calmette-Guérin (BCG)-activated murine peritoneal macrophages. Sheep erythrocytes (E) coated with IgG [E(IgG)], E coated with IgM and complement [E(IgM)C], and E treated with 1% glutaraldehyde (GE) were used as stimuli. All three types of macrophages released superoxide anion (O2-) during phagocytosis of E(IgG). All macrophage types phagocytosed E(IgM)C and GE but none were stimulated to release O2- during phagocytosis of these particles. Vigorous consumption of oxygen was also stimulated by the ingestion of E(IgG) but not by ingestion of E(IgM)C or GE. E(IgM)C did not scavenge the O2- released from macrophages during phagocytosis of E(IgG) or during exposure to phorbol myristate acetate, and further addition of IgG anti-E antibody to E(IgM)C or GE permitted optimal stimulation of macrophage O2- release by these particles. The capacity of macrophages to ingest E(IgM)C and GE without stimulating the respiratory burst raises the possibility that clearance of particulate matter not opsonized with specific IgG might be achieved without stimulation of the release of toxic oxygen metabolites, and, therefore, without the risk of oxidative damage to the phagocytic cell or surrounding tissue.
我们利用驻留的、脂多糖（LPS）诱导的以及卡介苗（BCG）激活的小鼠腹腔巨噬细胞，探讨了吞噬作用与氧化代谢触发之间的关系。用包被有IgG的绵羊红细胞（E）[E（IgG）]、包被有IgM和补体的E[E（IgM）C]以及用1%戊二醛（GE）处理的E作为刺激物。在吞噬E（IgG）的过程中，所有三种类型的巨噬细胞都会释放超氧阴离子（O2-）。所有类型的巨噬细胞都能吞噬E（IgM）C和GE，但在吞噬这些颗粒的过程中，没有一种巨噬细胞被刺激释放O2-。吞噬E（IgG）也会刺激氧气的大量消耗，但吞噬E（IgM）C或GE则不会。在吞噬E（IgG）或暴露于佛波酯肉豆蔻酸酯的过程中，E（IgM）C不会清除巨噬细胞释放的O2-，并且向E（IgM）C或GE中进一步添加抗E IgG抗体可使这些颗粒对巨噬细胞O2-释放产生最佳刺激。巨噬细胞摄取E（IgM）C和GE而不刺激呼吸爆发的能力增加了一种可能性，即清除未被特异性IgG调理的颗粒物时，可能不会刺激有毒氧代谢产物的释放，因此，不会有对吞噬细胞或周围组织造成氧化损伤的风险。
