Gnaedinger J M, Noronha A B, Druse M J
J Neurochem. 1984 May;42(5):1281-5. doi: 10.1111/j.1471-4159.1984.tb02784.x.
The present study examined myelin gangliosides in the developing offspring of rats that were pair-fed control or ethanol liquid diets prior to and during gestation. Between 17 and 31 days of age, we observed an increase in the proportion of GM1 in myelin (from 15% to 38% of ganglioside sialic acid) and a decrease in the proportion of GT1b (from 26% to 4%). GM4 was detected at all ages examined. Between 17 and 31 days of age, there was an increase in the proportion of N-acetylmannosamine-derived radioactivity associated with GM1 (from 16% to 22%) and GM4 (from 5% to 13%), and a decrease in that associated with GT1b (from 24% to 4%). Small, but significant (p less than 0.05), developmentally related differences were found in GD2 and GD3. Detection of GM4 in myelin of young rats in the present study appears to depend on the use of nonpartitioning methods of ganglioside extraction. Although the distribution of myelin gangliosides and radioactivity was near-normal in ethanol-treated pups, there was a consistent decrease in the proportion and radioactivity associated with the major myelin ganglioside, GM1.
本研究检测了在妊娠前及妊娠期间成对饲喂对照或乙醇液体饲料的大鼠发育中后代的髓鞘神经节苷脂。在17至31日龄之间，我们观察到髓鞘中GM1的比例增加（从神经节苷脂唾液酸的15%增至38%），而GT1b的比例降低（从26%降至4%）。在所有检测的年龄段均检测到GM4。在17至31日龄之间，与GM1相关的N - 乙酰甘露糖胺衍生放射性比例增加（从16%增至22%），与GM4相关的比例增加（从5%增至13%），而与GT1b相关的比例降低（从24%降至4%）。在GD2和GD3中发现了微小但显著的（p小于0.05）与发育相关的差异。本研究中在幼鼠髓鞘中检测到GM4似乎取决于神经节苷脂提取的非分配方法。尽管乙醇处理的幼崽中髓鞘神经节苷脂和放射性的分布接近正常，但与主要髓鞘神经节苷脂GM1相关的比例和放射性持续降低。
