Mizobe F, Iwamoto M, Livett B G
J Neurochem. 1984 May;42(5):1433-8. doi: 10.1111/j.1471-4159.1984.tb02805.x.
A pharmacological study was made of the effects of veratridine and lasalocid on the release of catecholamines, acetylcholinesterase (AChE) and dopamine-beta-hydroxylase (DBH) from cultures of isolated bovine adrenal chromaffin cells. Exposure of the cultures to veratridine resulted in concomitant release of catecholamines and AChE into the external medium in a dose-dependent and Ca2+-dependent manner. A Ca2+ ionophore, lasalocid, also produced a dose-dependent and parallel release of both catecholamines and AChE. The release of the two components was accompanied by release of DBH. The present results provide pharmacological evidence for a parallel release of catecholamines, AChE, and DBH from cultured adrenal chromaffin cells, and the stoichiometry of the release evoked by different secretagogues suggests that AChE and catecholamines are released from different cellular compartments.
对藜芦定和拉沙洛西对离体牛肾上腺嗜铬细胞培养物中儿茶酚胺、乙酰胆碱酯酶（AChE）和多巴胺-β-羟化酶（DBH）释放的影响进行了药理学研究。将培养物暴露于藜芦定导致儿茶酚胺和AChE以剂量依赖性和Ca2+依赖性方式同时释放到细胞外培养基中。一种Ca2+离子载体，拉沙洛西，也产生了儿茶酚胺和AChE的剂量依赖性平行释放。这两种成分的释放伴随着DBH的释放。目前的结果为培养的肾上腺嗜铬细胞中儿茶酚胺、AChE和DBH的平行释放提供了药理学证据，并且不同促分泌剂引起的释放化学计量表明AChE和儿茶酚胺从不同的细胞区室释放。
