Iwasa K, Oomori Y, Tanaka H
Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Rakuno Gakuen University, Hokkaido, Japan.
J Vet Med Sci. 1999 Jun;61(6):621-9. doi: 10.1292/jvms.61.621.
The present study showed the acetylcholinesterase (AChE) activity, and neurofilament protein (NFP), catecholamine-synthesizing enzymes, dopamine beta-hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) immunoreactivities in the mouse adrenal gland during postnatal development. From birth to postnatal-1-day, AChE activity was weakly and diffusely found in some medullary cells and in very few nerve fibers whereas strong NFP immunoreactivity was seen in a few ganglion cells and in remarkably numerous nerve fibers in the medulla. Almost all meduallary cells were reactive for both DBH and PNMT during this period. From postnatal-2- or -3-day to postnatal-1-week, strong AChE activity was observed in a few large ganglion cells, but the reaction was weak in clusters of chromaffin cells, and the number of strong AChE-active nerve fibers in the medulla was rapidly increased. From postnatal-2-day onwards, the number of NFP-immunoreactive nerve fibers in the medulla were remarkably numerous. Numerous chromaffin cells were reactive for both DBH and PNMT whereas some chromaffin cells were reactive for only DBH from postnatal-2-day onwards. These results suggest that drastic changes such as an increase of acetylcholine in the nerve fibers, differentiation of noradrenaline and adrenaline cells of the medulla may occur during this period. From postnatal-2-week to postnatal-3-week, weak AChE activity was seen in the clusters of several chromaffin cells and a few ganglion cells, and the number of AChE-active nerve fibers in the medulla was gradually increased. From postnatal-4-week to postnatal-8-week (adult), the distribution and frequency of AChE activity in the adrenal gland were similar to those at postnatal-3-week. In the adult, AChE activity was weakly seen in the clusters of several chromaffin cells showing noradrenaline fluorescence in the adrenal medulla. The noradrenaline cells were contacted by denser AChE-reactive nerve fibers than adrenaline cells. These results suggest that the development of cholinergic nervous system in the mouse adrenal medulla may be completed by postnatal-3-week.
本研究显示了小鼠肾上腺在出生后发育过程中乙酰胆碱酯酶（AChE）活性、神经丝蛋白（NFP）、儿茶酚胺合成酶、多巴胺β-羟化酶（DBH）和苯乙醇胺N-甲基转移酶（PNMT）的免疫反应性。从出生到出生后1天，在一些髓质细胞和极少数神经纤维中可微弱且弥散地发现AChE活性，而在少数神经节细胞和髓质中大量的神经纤维中可见强烈的NFP免疫反应性。在此期间，几乎所有髓质细胞对DBH和PNMT均有反应。从出生后2或3天到出生后1周，在少数大神经节细胞中观察到强烈的AChE活性，但在嗜铬细胞簇中的反应较弱，且髓质中具有强AChE活性的神经纤维数量迅速增加。从出生后2天起，髓质中NFP免疫反应性神经纤维数量显著增多。许多嗜铬细胞对DBH和PNMT均有反应，而从出生后2天起，一些嗜铬细胞仅对DBH有反应。这些结果表明，在此期间可能会发生诸如神经纤维中乙酰胆碱增加、髓质中去甲肾上腺素和肾上腺素细胞分化等剧烈变化。从出生后2周到出生后3周，在几个嗜铬细胞簇和少数神经节细胞中可见微弱的AChE活性，且髓质中AChE活性神经纤维数量逐渐增加。从出生后4周到出生后8周（成年期），肾上腺中AChE活性的分布和频率与出生后3周时相似。在成年期，在肾上腺髓质中显示去甲肾上腺素荧光的几个嗜铬细胞簇中可微弱地看到AChE活性。与肾上腺素细胞相比，去甲肾上腺素细胞与更密集的AChE反应性神经纤维接触。这些结果表明，小鼠肾上腺髓质中胆碱能神经系统的发育可能在出生后3周完成。
