Shargel L, Banijamali A R, Kuttab S H
J Pharm Sci. 1984 Feb;73(2):161-4. doi: 10.1002/jps.2600730206.
The rate of reduction was determined for a variety of azo dyes using the rat hepatic azoreductase enzyme system. In decreasing order, the rates of reduction for the azo dyes expressed as nmol of arylamine product formed/min/0.25 g of liver were amaranth (33.2), azosulfamide (32.5), orange G (12.4), 1,2-dimethyl-4-p-(carboxyphenylazo)-5-hydroxybenzene (CPA) (9.27), brilliant crystal scarlet (8.00), sulfachrysoidine (7.27), and Sudan I (1.03). A comparison of the partition coefficient with its rate of reduction indicated that the water-soluble azo dyes were reduced more rapidly than the lipid-soluble ones. Furthermore, higher rates of reduction were observed for those dyes containing electron-withdrawing groups on the aromatic rings.
使用大鼠肝脏偶氮还原酶系统测定了多种偶氮染料的还原速率。以每分钟每0.25克肝脏形成的芳胺产物的纳摩尔数表示，偶氮染料的还原速率从高到低依次为：苋菜红（33.2）、偶氮磺胺（32.5）、橙黄G（12.4）、1,2 - 二甲基 - 4 - 对 - （羧基苯基偶氮） - 5 - 羟基苯（CPA）（9.27）、亮晶猩红色（8.00）、磺胺二甲基嘧啶（7.27）和苏丹红I（1.03）。分配系数与其还原速率的比较表明，水溶性偶氮染料比脂溶性偶氮染料还原得更快。此外，对于那些在芳环上含有吸电子基团的染料，观察到了更高的还原速率。
