Menon G, Norris B, Webster J
J Pharm Sci. 1984 Feb;73(2):251-3. doi: 10.1002/jps.2600730227.
A high-performance liquid chromatographic method has been developed for the simultaneous determination of chloroprocaine hydrochloride and its hydrolytic degradation product, 4-amino-2-chlorobenzoic acid. Separation is achieved using a mu-Bondapak C18 column and the eluant, water-acetonitrile-methanol-glacial acetic acid (74:20:5:1) containing 0.05-0.08% (w/v) sodium 1-heptanesulfonate, at a flow rate of 2 mL/min. Benzoic acid and p-nitroacetophenone were used as internal standards. A variable-wavelength UV detector (278 nm) was used for detection of the compounds. The method is simple, accurate, and precise.
已开发出一种高效液相色谱法，用于同时测定盐酸氯普鲁卡因及其水解降解产物4-氨基-2-氯苯甲酸。使用μ-Bondapak C18柱进行分离，洗脱液为含有0.05 - 0.08%（w/v）1-庚烷磺酸钠的水 - 乙腈 - 甲醇 - 冰醋酸（74:20:5:1），流速为2 mL/min。苯甲酸和对硝基苯乙酮用作内标。使用可变波长紫外检测器（278 nm）检测这些化合物。该方法简便、准确且精密。
