高效液相色谱法同时测定丁卡因及其降解产物对正丁氨基苯甲酸

Simultaneous determination of tetracaine and its degradation product, p-n-butylaminobenzoic acid, by high-performance liquid chromatography.

作者信息

Menon G N, Norris B J

出版信息

J Pharm Sci. 1981 May;70(5):569-70. doi: 10.1002/jps.2600700527.

Abstract

A high-performance liquid chromatographic method was developed for the simultaneous determination of tetracaine hydrochloride and its hydrolytic degradation product, p-n-butylaminobenzoic acid. Separation was achieved using a mu Bondapak C18 column and the eluent, water-acetonitrile-methanol (60:20:20), containing 0.06% (v/v) sulfuric acid, 0.5% (w/v) sodium sulfate, and 0.02% (w/v) sodium heptanesulfonate, at a flow rate of 2 ml/min Salicylic acid and propiophenone were used as internal standards. The UV detector response at 305 nm was linear for tetracaine hydrochloride in the 0.4--2.0-mg/ml range and for p-n-butylaminobenzoic acid in the 0.003--0.02-mg/ml range. The method is simple and precise.

摘要

建立了一种高效液相色谱法，用于同时测定盐酸丁卡因及其水解降解产物对正丁基氨基苯甲酸。采用μ Bondapak C18柱，以水 - 乙腈 - 甲醇（60:20:20）为洗脱液，其中含有0.06%（v/v）硫酸、0.5%（w/v）硫酸钠和0.02%（w/v）庚烷磺酸钠，流速为2 ml/min，以水杨酸和苯丙酮作为内标。在305 nm处，紫外检测器对盐酸丁卡因在0.4 - 2.0 mg/ml范围内、对正丁基氨基苯甲酸在0.003 - 0.02 mg/ml范围内的响应呈线性。该方法简单且精确。