Vogh B P, Godman D R
J Pharmacol Exp Ther. 1984 Apr;229(1):207-9.
In a system for ventriculocisternal perfusion of the choroid plexus, the rate of formation of new cerebrospinal fluid was measured by changes in dilution of an impermeant dye in the perfusate. Norepinephrine added to the perfusate decreased formation of cerebrospinal fluid in rats as was previously demonstrated in rabbits. The dose-response relationship for rats was determined. The formation rate was decreased 42% by 10(-3) M norepinephrine. Acetazolamide, 50 mg/kg i.v., caused a decrease of 46%. Given together, these drugs decreased formation 79%, demonstrating essentially full addition between the regulatory mechanisms involved. Addition of equal magnitude occurred when intraventricular nialamide, an inhibitor of monoamine oxidase, and i.v. acetazolamide were given together. This demonstrates addition between acetazolamide and endogenous norepinephrine (or other catecholamines present) in which metabolic breakdown is prevented by the inhibitor. The degree of reduction in cerebrospinal fluid formation seen in these experiments exceeds that reported for numerous other trials of single drugs.
在一个用于脉络丛脑室池灌注的系统中，通过灌注液中一种非渗透性染料稀释度的变化来测量新脑脊液的生成速率。如先前在兔子身上所证实的那样，向灌注液中添加去甲肾上腺素会降低大鼠脑脊液的生成。确定了大鼠的剂量 - 反应关系。10(-3)M去甲肾上腺素使生成速率降低了42%。静脉注射50mg/kg乙酰唑胺导致生成速率降低46%。同时给予这两种药物时，生成速率降低了79%，这表明所涉及的调节机制之间基本上是完全相加的。当静脉注射单胺氧化酶抑制剂尼拉米和静脉注射乙酰唑胺同时给予时，也出现了同等程度的相加作用。这表明乙酰唑胺与内源性去甲肾上腺素（或存在的其他儿茶酚胺）之间存在相加作用，其中抑制剂阻止了代谢分解。这些实验中观察到的脑脊液生成减少程度超过了许多其他单一药物试验所报告的程度。
