Kitraki E, Alexis M N, Stylianopoulou F
J Steroid Biochem. 1984 Jan;20(1):263-9. doi: 10.1016/0022-4731(84)90215-2.
Dexamethasone receptors were measured by conventional equilibrium steroid binding studies in rat liver and brain cytosol, during late prenatal and postnatal development, Receptor binding could be detected in both cytosol preparations as early as the 17th day of gestation. Receptor levels in the cytosol from intact animals reached adult values by the 1st day after birth in both tissues. Using adrenalectomized animals an increase which reached adult values was observed during the first postnatal week for liver and the second postnatal week for brain. At physiological concentrations of endogenous glucocorticoids depletion of receptor from the cytosol of intact animals was minimal at postnatal day 1 and reached adult levels by day 7. Chromatographic analysis in DEAE-Sephadex A50 minicolumns of unactivated and activated receptor constituents revealed the same pattern as that of adult animals. Glucocorticoid receptor complex from developing liver and brain was shown to be capable of binding to isolated adult liver nuclei after in vitro activation. However full capacity, for nuclear binding was observed in vivo, after injection of inducing doses of [3H]dexamethasone: By the end of the first week after birth adult nuclear binding capacity was observed in experiments in vivo while values peaked during the second week, in both tissues studied.
在产前后期和产后发育期间，通过常规平衡类固醇结合研究测定大鼠肝脏和脑细胞溶胶中的地塞米松受体。早在妊娠第17天，在两种细胞溶胶制剂中均可检测到受体结合。完整动物细胞溶胶中的受体水平在出生后第1天在两种组织中均达到成年值。使用肾上腺切除的动物，观察到肝脏在出生后第一周和大脑在出生后第二周增加到成年值。在内源性糖皮质激素的生理浓度下，完整动物细胞溶胶中受体的耗竭在出生后第1天最小，并在第7天达到成年水平。在DEAE-葡聚糖A50微柱上对未活化和活化的受体成分进行色谱分析，显示出与成年动物相同的模式。发育中的肝脏和大脑中的糖皮质激素受体复合物在体外活化后显示能够与分离的成年肝脏细胞核结合。然而，在注射诱导剂量的[3H]地塞米松后，在体内观察到了完全的核结合能力：在出生后第一周结束时，在体内实验中观察到成年核结合能力，而在研究的两种组织中，值在第二周达到峰值。
