Ribeiro S M, Armelin H A
Mol Cell Biochem. 1984;59(1-2):173-81. doi: 10.1007/BF00231313.
The effects of Mg2+ and Ca2+ deprivation on survival and growth of normal and transformed cultured cells were studied. Normal fibroblast from several origins (mouse, hamster and human) did not grow in 2 microM Mg2+ medium, whereas transformed fibroblasts (mouse and rat) and tumor cells of other types (mouse adrenocortical and rat glial cells) grew optimal. All transformed or tumorogenic cells that showed low growth requirement for Mg2+ were able to develop colonies in agarose suspension cultures, i.e., were anchorage-independent fo growth. Mg2+ deprivation of normal cells did not lead to cell cycle arresting at G0/G1 and cell death accounts for the limited growth of these cells in medium containing low Mg2+ concentration. Contrary to Mg2+, starvation for serum or for Ca2+ caused normal cells to undergo cell cycle arrest at the G0/G1 phase. During the progressive spontaneous transformation of Swiss mouse 3T3 fibroblasts, the decrease in growth requirements for Mg2+ and Ca2+ do not occur at the same time. The requirement for external Ca2+ lowers before the onset of anchorage-independent growth while the Mg2+ requirement only decreases after cells become anchorage-independent. Therefore the reductions in growth requirement for Mg2+ and Ca2+ that take place in cell transformation are not linked events.
研究了镁离子（Mg2+）和钙离子（Ca2+）缺乏对正常及转化培养细胞存活和生长的影响。来自多个来源（小鼠、仓鼠和人类）的正常成纤维细胞在2微摩尔/升Mg2+培养基中无法生长，而转化的成纤维细胞（小鼠和大鼠）以及其他类型的肿瘤细胞（小鼠肾上腺皮质细胞和大鼠神经胶质细胞）生长良好。所有对Mg2+生长需求较低的转化或致瘤细胞都能够在琼脂糖悬浮培养中形成集落，即不依赖贴壁生长。正常细胞缺乏Mg2+不会导致细胞周期停滞在G0/G1期，细胞死亡是这些细胞在低Mg2+浓度培养基中生长受限的原因。与Mg2+相反，血清饥饿或Ca2+饥饿会使正常细胞在G0/G1期发生细胞周期停滞。在瑞士小鼠3T3成纤维细胞逐渐自发转化过程中，对Mg2+和Ca2+的生长需求降低并非同时发生。在不依赖贴壁生长开始之前，对外部Ca2+的需求降低，而对Mg2+的需求仅在细胞变得不依赖贴壁后才降低。因此，细胞转化过程中对Mg2+和Ca2+生长需求的降低并非相关事件。
