Balk S D
Proc Natl Acad Sci U S A. 1971 Feb;68(2):271-5. doi: 10.1073/pnas.68.2.271.
In a culture medium of low calcium concentration containing 5% heat-inactivated chicken plasma, normal chicken fibroblasts divide very slowly, while their counterparts transformed with Schmidt-Ruppin Rous sarcoma virus divide much more rapidly. In the same low-calcium medium, the use of heat-inactivated serum, rather than heat-inactivated plasma, results in rapid division of both normal and transformed cells. The use of heat-inactivated plasma rather than serum, and the use of a low-calcium medium, combine to permit the demonstration of a marked difference between the proliferative behaviors of normal and neoplastic cells and suggest a basis for the autonomy of the latter.
在含有5%热灭活鸡血浆的低钙浓度培养基中，正常鸡成纤维细胞分裂非常缓慢，而用施密特-鲁平劳氏肉瘤病毒转化的对应细胞分裂则快得多。在相同的低钙培养基中，使用热灭活血清而非热灭活血浆会导致正常细胞和转化细胞都快速分裂。使用热灭活血浆而非血清，以及使用低钙培养基，共同使得能够证明正常细胞和肿瘤细胞增殖行为之间的显著差异，并为后者的自主性提供了一个基础。
