小鼠乳酸脱氢酶（LDH）C4（睾丸型）与LDH A4（肌肉型）和LDH B4（心脏型）存在免疫化学交叉反应。

Mouse lactate dehydrogenase (LDH) C4 (testis) is immunochemically cross-reactive with LDH A4 (muscle) and LDHB4 (heart).

作者信息

Wright L L, Swofford J H

出版信息

Scand J Immunol. 1984 Mar;19(3):247-54. doi: 10.1111/j.1365-3083.1984.tb00926.x.

DOI:10.1111/j.1365-3083.1984.tb00926.x

Abstract

It has been reported that lactate dehydrogenase (LDH) purified from testes (LDH C) of homotherms is not immunochemically cross-reactive with somatic forms of LDH purified from heart (LDH B) or muscle (LDH A). On the basis of this premise, LDH C has been considered for use as a contraceptive vaccine. Data presented here indicate that mouse antisera to either mouse or rat LDH C are cross-reactive with LDH A and B purified from muscle and heart tissues of mice. However, rabbit antisera to mouse LDH C are not cross-reactive with either mouse LDH A or B. Thus, the degree of cross-reactivity is dependent on the species from which the immunogen LDH is purified, the antisera are derived, and the LDH used in the assay is purified. The determination that LDH A, B, and C are immunochemically cross-reactive is of importance in evaluating LDH C as an immunogen in an immunologic approach to contraception.

摘要

据报道，从恒温动物睾丸（LDH C）中纯化的乳酸脱氢酶与从心脏（LDH B）或肌肉（LDH A）中纯化的体细胞形式的乳酸脱氢酶没有免疫化学交叉反应。基于这一前提，LDH C已被考虑用作避孕疫苗。此处呈现的数据表明，针对小鼠或大鼠LDH C的小鼠抗血清与从小鼠肌肉和心脏组织中纯化的LDH A和B有交叉反应。然而，针对小鼠LDH C的兔抗血清与小鼠LDH A或B均无交叉反应。因此，交叉反应的程度取决于纯化免疫原LDH的物种、抗血清的来源以及测定中使用的纯化LDH。确定LDH A、B和C具有免疫化学交叉反应对于评估LDH C作为免疫避孕方法中的免疫原具有重要意义。