编码小鼠精子特异性乳酸脱氢酶C的cDNA在中国仓鼠卵巢细胞中的表达。
Expression of the cDNA encoding for mouse sperm-specific lactate dehydrogenase C in Chinese-hamster ovary cells.
作者信息
Hou E W, Sakai I, Li S S
机构信息
Laboratory of Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.
出版信息
Biochem J. 1987 Oct 1;247(1):241-4. doi: 10.1042/bj2470241.
The cloned cDNA encoding for mouse sperm-specific lactate dehydrogenase C (LDH-C) was inserted immediately downstream to the MMTV 5' LTR promoter, and it was shown to synthesize mouse LDH-C polypeptide in Chinese-hamster ovary cells. The mouse LDH-C subunit and the endogenous Chinese-hamster LDH-A subunit formed in vivo a heterotetrameric LDH-A3C1 isoenzyme, and this novel isoenzyme exhibited enzymic activity utilizing lactate as substrate.
编码小鼠精子特异性乳酸脱氢酶C（LDH-C）的克隆cDNA被插入到MMTV 5' LTR启动子的紧下游，并显示其在中国仓鼠卵巢细胞中合成小鼠LDH-C多肽。小鼠LDH-C亚基和内源性中国仓鼠LDH-A亚基在体内形成了一种异源四聚体LDH-A3C1同工酶，这种新型同工酶表现出以乳酸为底物的酶活性。
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