Shimizu A, Ogata T, Kitaoka M
Intervirology. 1977;8(1):52-9. doi: 10.1159/000148877.
A comparative analysis was performed on two substrains, c-1 and c-3, derived from the Nakayama-NIH strain of Japanese encephalitis virus. The infectivity of the two substrains was inactivated at similar rates by treatment with Tween 80 and Tween 20 but not by treatment with Tween 60. However, hemagglutinating activity as measured at pH 6.8 of the c-3 substrain treated with Tween 80 was more labile than that of the c-1 substrain treated with Tween 80. Also, the immunogenicity (neutralizing and hemagglutination-inhibiting antibodies) was different between the two substrains in sensitivity to low pH (6.8) and to Tween 80. With the c-1 substrain a combination of these treatments reduced the immunogenicity, but each treatment applied singly did not materially reduce it. The immunogenicity of the c-3 substrain was reduced by single as well as combined applications of the two treatments. Evidence was obtained which suggested that the part of the antigen sensitive to these treatments was strain-specific, whereas the resistant antigen was common among the Japanese encephalitis-St. Louis encephalitis complex members.
对源自日本脑炎病毒中山 - 美国国立卫生研究院（Nakayama - NIH）毒株的两个亚株c - 1和c - 3进行了比较分析。用吐温80和吐温20处理时，这两个亚株的感染性以相似的速率失活，但用吐温60处理时则不然。然而，在pH 6.8下测量，用吐温80处理的c - 3亚株的血凝活性比用吐温80处理的c - 1亚株更不稳定。此外，在对低pH（6.8）和吐温80的敏感性方面，两个亚株的免疫原性（中和抗体和血凝抑制抗体）有所不同。对于c - 1亚株，这些处理的组合降低了免疫原性，但单独应用每种处理并没有实质性地降低它。c - 3亚株的免疫原性在单独以及联合应用这两种处理时均降低。有证据表明，对这些处理敏感的抗原部分是菌株特异性的，而抗性抗原在日本脑炎 - 圣路易斯脑炎复合体成员中是常见的。
