Swanson J L, Issitt C H, Mann E W, Condie R M, Simmons R L, McCullough J
Transfusion. 1984 Mar-Apr;24(2):141-3. doi: 10.1046/j.1537-2995.1984.24284173345.x.
Passively acquired hemagglutinating antibodies may be detected in the serum of patients receiving horse anti-lymphoblast globulin (ALG) or anti-thymocyte globulin (ATG). Thirty-seven patients receiving ALG following renal transplantation were studied. Eight patients monitored daily all developed positive direct antiglobulin tests (DAT) and positive red cell antibody screening tests. Fifteen of 32 recipients developed red cell antibodies reactive at room temperature in saline, 4 of 32 in albumin at 37 degrees C, and 33 of 37 in the antiglobulin test. Horse globulin was detected on the red cells of all six recipients tested with rabbit anti-horse globulin. Ether eluates prepared from the red cells of 20 patients showed no specificity for common red cell antigens. Anti-human globulin (AHG), absorbed with ALG-coated red cells to remove the component in the AHG which was crossreacting with horse globulin, was used successfully for antibody screening and identification, direct antiglobulin testing, and/or the antiglobulin crossmatching of 27 ALG and ATG recipients, including five with red cell alloantibodies.
在接受马抗淋巴细胞球蛋白（ALG）或抗胸腺细胞球蛋白（ATG）治疗的患者血清中可检测到被动获得的血凝抗体。对37例肾移植后接受ALG治疗的患者进行了研究。8例每日监测的患者直接抗球蛋白试验（DAT）均呈阳性，红细胞抗体筛查试验也呈阳性。32例受者中有15例在室温盐水介质中出现红细胞抗体反应性，32例中有4例在37℃白蛋白介质中出现反应性，37例中有33例在抗球蛋白试验中出现反应性。用兔抗马球蛋白检测的所有6例受者的红细胞上均检测到马球蛋白。从20例患者红细胞制备的乙醚洗脱液对常见红细胞抗原无特异性。用包被ALG的红细胞吸收抗人球蛋白（AHG）以去除AHG中与马球蛋白发生交叉反应的成分，成功用于27例ALG和ATG受者的抗体筛查和鉴定、直接抗球蛋白试验及/或抗球蛋白交叉配血，其中包括5例有红细胞同种抗体的患者。
