Goyal H O, Vig M M
Am J Vet Res. 1984 Mar;45(3):444-50.
The histochemical localization of alkaline phosphatase (ALP) and acid phosphatase (ACP) was determined in regions I to VI of the epididymis in mature intact, orchidectomized, and orchidectomized testosterone-treated bulls. The intensity of ALP activity was essentially the same in all regions; however, its localization varied depending upon the region. Although the stereocilia and luminal border of epithelial cells were strongly-positive in regions I to III, these were negative in regions IV to VI. The basement membrane and circumtubular smooth muscle cells were strongly ALP reactive in all regions. Epithelial ALP activity was abolished completely by orchidectomy; however, it was restored to normal concentration by testosterone treatment implying its dependence on circulating testosterone. The ACP activity was present in the epithelial cells of all regions with the strongest activity in the supranuclear region. Similar to ALP, ACP activity was markedly reduced in the epithelial cells by orchidectomy. However, in contrast to ALP activity, lost ACP activity was only minimally increased by testosterone treatment in all regions, except in region VI where it was restored to a normal concentration. These observations imply that although the epithelial ACP activity of region VI was mainly under the influence of circulatory testosterone, there may be other factors such as luminal androgens, testicular fluid, and sperm that may be important in regulating the ACP activity of regions I to V of the epididymis. The importance of ALP and ACP in the epididymal epithelium was discussed.
在成熟未阉割、阉割及阉割后用睾酮处理的公牛附睾的I至VI区，测定了碱性磷酸酶（ALP）和酸性磷酸酶（ACP）的组织化学定位。所有区域的ALP活性强度基本相同；然而，其定位因区域而异。虽然I至III区上皮细胞的静纤毛和管腔边界呈强阳性，但IV至VI区则为阴性。所有区域的基底膜和管周平滑肌细胞对ALP均有强烈反应。阉割后上皮ALP活性完全消失；然而，睾酮处理可使其恢复至正常浓度，这表明其依赖于循环睾酮。ACP活性存在于所有区域的上皮细胞中，核上区域活性最强。与ALP相似，阉割后上皮细胞中的ACP活性明显降低。然而，与ALP活性不同的是，除VI区恢复至正常浓度外，所有区域睾酮处理仅使丧失的ACP活性略有增加。这些观察结果表明，虽然VI区上皮ACP活性主要受循环睾酮的影响，但可能还有其他因素，如管腔雄激素、睾丸液和精子，对调节附睾I至V区的ACP活性可能很重要。文中讨论了ALP和ACP在附睾上皮中的重要性。
