Stipkovits L, Vuillaume A, Rousselot D, Larrue P, Bové J M
Ann Microbiol (Paris). 1984 Jan-Feb;135A(1):91-4. doi: 10.1016/s0769-2609(84)80063-0.
Several mycoplasma isolation trials were performed on infertile goose eggs and goose embryos which died during incubation, as well as on geese of different ages. A total of 43 out of 110 goose eggs proved to be contaminated by mycoplasmas. Upon autopsy of birds which laid positive eggs, lesions were observed in the airsacs. Mycoplasmas could be isolated from their air sacs and oviduct. Four out of 15 strains examined biochemically and serologically with antisera prepared against all known avian mycoplasma species were identified as Acholeplasma laidlawii and A. axanthum, respectively. Two strains proved to be glucose-positive and arginine-negative and 9 were glucose-negative but arginine-positive. Some strains caused 50-80% mortality among embryos inoculated intra-yolk-sac at 12 days. In goslings inoculated at the age of 3 days with these strains, we observed fibrinous airsacculitis and peritonitis. By inoculating laying geese with one of the strains, we demonstrated decreasing egg production, increasing early-embryo mortality and egg transmission of mycoplasmas.
对不育鹅蛋、孵化期间死亡的鹅胚胎以及不同年龄的鹅进行了多次支原体分离试验。110枚鹅蛋中共有43枚被证明受到支原体污染。对产下阳性蛋的鹅进行解剖时，在气囊中观察到病变。可从它们的气囊和输卵管中分离出支原体。在对15株菌株进行生化和血清学检测时，分别用针对所有已知禽支原体物种制备的抗血清进行检测，其中4株被鉴定为莱氏无胆甾原体和黄色无胆甾原体。2株菌株葡萄糖阳性但精氨酸阴性，9株葡萄糖阴性但精氨酸阳性。一些菌株在12日龄时通过卵黄囊接种，导致接种胚胎死亡率达到50%-80%。在3日龄雏鹅中接种这些菌株后，我们观察到了纤维素性气囊炎和腹膜炎。通过用其中一种菌株接种产蛋鹅，我们证明了产蛋量下降、早期胚胎死亡率增加以及支原体的蛋传播。
