Underberg W J
J Pharm Sci. 1978 Aug;67(8):1131-3. doi: 10.1002/jps.2600670827.
Methods for the determination of promethazine and several degradation products, which can be used in kinetic studies, were developed. All determinations were carried out after isolation of the compounds by TLC. Promethazine was determined by oxidation with vanadyl sulfate in an acidic medium. The method was suitable for approximately 3 mg of the compound. 3H-Phenothiazine-3-one was determined spectrophotometrically at 492 nm in acetone. The compound could be determined in amounts of 30-40 microgram. Promethazine 5-oxide was determined spectrophotometrically using the acid dye method; 100-200 microgram could be determined. 7-Hydroxy-3H-phenothiazine-3-one could be determined spectrophotometrically at 600 nm after extraction of the other compounds from the solution. The medium had to be alkaline and contain water in a fixed ratio. The method was suitable for 15-30 microgram of the compound.
开发了可用于动力学研究的测定异丙嗪及其几种降解产物的方法。所有测定均在通过薄层色谱法分离化合物后进行。异丙嗪在酸性介质中用硫酸氧钒氧化进行测定。该方法适用于约3mg的该化合物。3H-吩噻嗪-3-酮在丙酮中于492nm处用分光光度法测定。该化合物的测定量可为30 - 40微克。5-氧化异丙嗪用酸性染料分光光度法测定；可测定100 - 200微克。从溶液中萃取出其他化合物后，7-羟基-3H-吩噻嗪-3-酮可在600nm处用分光光度法测定。介质必须呈碱性且含有固定比例的水。该方法适用于15 - 30微克的该化合物。
