Zuleski F R, Loh A, Di Carlo F J
J Pharm Sci. 1978 Aug;67(8):1138-41. doi: 10.1002/jps.2600670829.
A radiochemical GLC analysis was developed for 3H-labeled ethinyl estradiol in human urine. The technique was applied to the unconjugated and aglycone fractions of urine collected from women who were dosed orally with: (a) single capsules containing 2.0 mg of 3H-quinestrol (900 muCi) and 2.5 mg of unlabeled quingestanol acetate dissolved in sesame oil and (b) single tablets containing 100 microgram of 3H-quinestrol (86 muCi). Unconjugated ethinyl estradiol in Day 1 urine collections represented means of 0.02% of the high quinestrol dose and 0.12% of the low dose. Ethinyl estradiol glucuronide in the same collections represented means of 0.55% of the high drug dose and 1.35% of the low dose. The method could detect 1-ng quantities of 3H-ethinyl estradiol and 3H-quinestrol.
已开发出一种放射化学气相色谱分析法，用于分析人尿中3H标记的炔雌醇。该技术应用于从口服给药的女性收集的尿液的未结合和苷元部分，这些女性口服了：(a) 含有2.0毫克3H炔诺酮（900微居里）和2.5毫克溶解在芝麻油中的未标记醋酸炔诺醇的单胶囊，以及(b) 含有100微克3H炔诺酮（86微居里）的单片剂。第1天尿液收集物中的未结合炔雌醇分别占高炔诺酮剂量的0.02%和低剂量的0.12%。同一收集物中的炔雌醇葡萄糖醛酸苷分别占高药物剂量的0.55%和低剂量的1.35%。该方法能够检测到1纳克量的3H-炔雌醇和3H-炔诺酮。
