Nitrous oxide reductase and the 120,000 MW copper protein of N2-producing denitrifying bacteria are different entities.

Zumft and Matsubara [1982) FEBS Lett. 148, 107-112) isolated a 120,000 MW copper protein from certain denitrifying bacteria which were capable of producing N2. The presence of this protein correlated with a nutritional requirement of copper for growth on and reduction of N2O by the bacteria. The copper protein was alleged by these workers to be nitrous oxide reductase. However, it is shown that the copper protein and nitrous oxide reductase have different molecular weights and exhibit different behavior upon anion exchange chromatography. The copper protein is therefore not nitrous oxide reductase.