Passarella S, Barile M, Atlante A, Quagliariello E
Biochem Biophys Res Commun. 1984 Mar 30;119(3):1039-46. doi: 10.1016/0006-291x(84)90879-9.
Reconstruction, using non-synaptosomal rat brain mitochondria, has been made here of the malate/oxaloacetate shuttle, made possible by the occurrence in rat brain mitochondria of a carrier mediated transport for oxaloacetate (Km = 60 microM), sensitive to dicarboxylate analogues and mersalyl, and of the two isoenzymes of malate dehydrogenase. Malate/oxaloacetate shuttle shows a high affinity for malate (Km = 100 microM) and Vmax = 40 nmoles NADH oxidized/min X mg mitochondrial protein at 20 degrees C. Its rate appears to depend on the activity of the malate/oxaloacetate exchange across the mitochondrial membrane. A possible role for the oxaloacetate carrier is proposed in aminoacid brain metabolism.
利用非突触体大鼠脑线粒体，对苹果酸/草酰乙酸穿梭进行了重建。大鼠脑线粒体中存在草酰乙酸的载体介导转运（Km = 60微摩尔），对二羧酸类似物和汞撒利敏感，且存在苹果酸脱氢酶的两种同工酶，这使得重建成为可能。苹果酸/草酰乙酸穿梭对苹果酸具有高亲和力（Km = 100微摩尔），在20℃时Vmax = 40纳摩尔NADH氧化/分钟×毫克线粒体蛋白。其速率似乎取决于线粒体膜上苹果酸/草酰乙酸交换的活性。草酰乙酸载体在氨基酸脑代谢中可能发挥作用。
