Almasio P, Piovella F, Ricetti M M, Samaden A, Semino G
Boll Soc Ital Biol Sper. 1984 Feb 28;60(2):225-30.
Cultured endothelium synthesizes an extracellular matrix that resembles the basement membrane of the vessels. We studied the aspect of extracellular matrices produced in presence or absence of dexamethasone by immunofluorescent technique for fibronectin; the glycoprotein is more abundant in matrices produced in the presence of dexamethasone than controls and forms a very dense meshwork. When endothelial cells resuspended in medium without FCS were seeded on these preformed matrices they adhered and replicated. The endothelium grown on matrices preformed in the presence of steroid showed a better morphology and covered a greater area indicating the presence of more abundant fibronectin content in basal lamina.
培养的内皮细胞合成一种类似于血管基底膜的细胞外基质。我们通过针对纤连蛋白的免疫荧光技术研究了在有或没有地塞米松的情况下产生的细胞外基质的情况；这种糖蛋白在有地塞米松存在时产生的基质中比在对照中更丰富，并形成非常致密的网络。当悬浮在无胎牛血清培养基中的内皮细胞接种在这些预先形成的基质上时，它们会黏附并增殖。在有类固醇存在的情况下预先形成的基质上生长的内皮细胞表现出更好的形态，覆盖的面积更大，这表明基底层中纤连蛋白含量更丰富。
