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Noncollagenous phosphoprotein derived from teleostean fish-scales.

作者信息

Sauk J J, Cocking-Johnson D, Cervenka V A, van Kampen C L

出版信息

Biochim Biophys Acta. 1984 Apr 10;798(2):199-203. doi: 10.1016/0304-4165(84)90304-0.

DOI:10.1016/0304-4165(84)90304-0
PMID:6712988
Abstract

A noncollagenous phosphoprotein derived from teleostean fish-scales is extracted by the use of a dissociative extraction scheme, utilizing 4 M guanidine-HCl, pH 7.4, followed by a 4 M guanidine-HCl, 0.5 M EDTA, pH 7.4. DEAE-cellulose chromatography and repeated Sepharose CL-6B chromatography are used to purify this protein. The isolated protein contains extensive quantities of aspartic and glutamic acids in addition to O-phosphoserine and O-phosphothreonine. The apparent molecular weight of the phosphoprotein as determined by Sepharose CL-6B chromatography is 13 000.

摘要

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