Giulian D
Brain Res. 1984 Mar 26;296(1):198-201. doi: 10.1016/0006-8993(84)90533-x.
Transected ganglion cells of the goldfish retina demonstrate a marked increase in protein biosynthesis as their axons regrow into a primary target tissue, the optic tectum. In order to examine what role the target tissue may play in regulating the pattern of neuronal protein biosynthesis, the tectum is removed at the time of axotomy. By 10 days after surgery, the production of specific polypeptides including the structural proteins, tubulin and actin, are not affected by tectal ablation. However, during a later phase of regeneration when axons would normally begin to re-connect with the tectum, the appearance of a radiolabeled polypeptide of 300 kDaltons is blocked by tectal ablation. These data suggest that ganglion cells are regulated by target tissues late in the regenerative process perhaps by contact between axons and cells of the tectum.
金鱼视网膜横断的神经节细胞在其轴突长入主要靶组织视顶盖时，蛋白质生物合成显著增加。为了研究靶组织在调节神经元蛋白质生物合成模式中可能起什么作用，在轴突切断时切除视顶盖。术后10天，包括结构蛋白微管蛋白和肌动蛋白在内的特定多肽的产生不受视顶盖切除的影响。然而，在再生的后期阶段，当轴突通常开始与视顶盖重新连接时，一种300千道尔顿放射性标记多肽的出现被视顶盖切除所阻断。这些数据表明，神经节细胞在再生过程后期受到靶组织的调节，可能是通过轴突与视顶盖细胞之间的接触实现的。
