Grover A K, Kwan C Y
Can J Physiol Pharmacol. 1984 Jan;62(1):76-9. doi: 10.1139/y84-011.
The rat vas deferens smooth muscle microsomes on isopycnic centrifugation gave two fractions, namely F2 (15-30% sucrose) and F3 (30-40% sucrose), with comparable ATP-dependent azide-insensitive Ca2+-uptake capacities, although these fractions differed from each other in various enzyme marker activities. The fractions F2 and F3 also show similar pH profiles for the ATP-independent and ATP-dependent Ca2+ uptake, and similar ionized Ca2+-concentration dependence for the ATP-dependent Ca2+ uptake. However, the fractions F2 and F3 differ from each other in that: (a) F3 shows higher permeability to Ca2+, and (b) F3 shows higher stimulation of the ATP-dependent Ca2+ uptake by oxalate. The F3 fraction can also be used to obtain membrane vesicles loaded with Ca2+ oxalate in the presence of ATP. However, the yield of the Ca2+ oxalate enriched fraction is too low to permit their further characterization.
大鼠输精管平滑肌微粒体在等密度离心时产生了两个组分，即F2（15 - 30%蔗糖）和F3（30 - 40%蔗糖），它们具有相当的依赖ATP的叠氮化物不敏感的Ca²⁺摄取能力，尽管这些组分在各种酶标记活性方面彼此不同。F2和F3组分在不依赖ATP和依赖ATP的Ca²⁺摄取方面也表现出相似的pH谱，并且在依赖ATP的Ca²⁺摄取方面表现出相似的离子化Ca²⁺浓度依赖性。然而，F2和F3组分在以下方面彼此不同：（a）F3对Ca²⁺的通透性更高，以及（b）F3对草酸盐刺激依赖ATP的Ca²⁺摄取的作用更强。在ATP存在的情况下，F3组分也可用于获得装载有草酸钙的膜囊泡。然而，富含草酸钙的组分的产量过低，无法对其进行进一步表征。
