在血管肌肉微粒体组分中，质膜成分是四氧嘧啶作用于ATP驱动的Ca2+转运的主要作用位点。
The plasma-membrane component is the primary site of action of alloxan on ATP-driven Ca2+ transport in vascular-muscle microsomal fractions.
作者信息
Kwan C Y
机构信息
Department of Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.
出版信息
Biochem J. 1988 Aug 15;254(1):293-6. doi: 10.1042/bj2540293.
Abstract
The direct effects of alloxan in vitro on the handling of Ca2+ by microsomal fractions from dog aortic smooth muscle were investigated. Preincubation of the vascular-muscle microsomal membranes with alloxan showed a suppression of both binding of Ca2+ (in the absence of ATP) and ATP-driven Ca2+ transport. Alloxan substantially inhibited the microsomal ATP-driven Ca2+ transport stimulated by Pi, but not that stimulated by oxalate. Studies using subfractions isolated from the microsomal membranes on a sucrose density gradient indicated that plasma membrane is the primary site of action of alloxan on the ATP-driven Ca2+ transport.
摘要
研究了四氧嘧啶在体外对犬主动脉平滑肌微粒体组分处理Ca2+的直接影响。用四氧嘧啶对血管肌肉微粒体膜进行预孵育，结果显示Ca2+结合（在无ATP的情况下）和ATP驱动的Ca2+转运均受到抑制。四氧嘧啶显著抑制了由Pi刺激的微粒体ATP驱动的Ca2+转运，但对草酸盐刺激的Ca2+转运没有抑制作用。利用在蔗糖密度梯度上从微粒体膜分离的亚组分进行的研究表明，质膜是四氧嘧啶对ATP驱动的Ca2+转运作用的主要位点。
相似文献
1
The plasma-membrane component is the primary site of action of alloxan on ATP-driven Ca2+ transport in vascular-muscle microsomal fractions.在血管肌肉微粒体组分中，质膜成分是四氧嘧啶作用于ATP驱动的Ca2+转运的主要作用位点。
Biochem J. 1988 Aug 15;254(1):293-6. doi: 10.1042/bj2540293.
2
Mechanism of inhibition by alloxan of ATP-driven calcium transport by vascular smooth muscle microsomes.四氧嘧啶对血管平滑肌微粒体ATP驱动的钙转运的抑制机制。
J Bioenerg Biomembr. 1988 Aug;20(4):517-31. doi: 10.1007/BF00762207.
3
In vitro inhibition of calcium binding by alloxan and of calcium transport by isolated vascular smooth muscle microsomes.
Can J Physiol Pharmacol. 1987 Nov;65(11):2346-9. doi: 10.1139/y87-371.
4
Subcellular origin of the oxalate- or inorganic phosphate-stimulated Ca2+ transport by smooth muscle microsomes: revisitation of the old problem by a new approach using saponin.
Biochim Biophys Acta. 1985 Sep 25;819(1):148-52. doi: 10.1016/0005-2736(85)90206-8.
5
Selective inhibition of oxalate-stimulated Ca2+ transport by cyclopiazonic acid and thapsigargin in smooth muscle microsomes.环匹阿尼酸和毒胡萝卜素对草酸刺激的平滑肌微粒体中Ca2+转运的选择性抑制作用。
Can J Physiol Pharmacol. 1996 Feb;74(2):182-92.
6
An analytical approach to the preparation and characterization of subcellular membranes from canine mesenteric arteries.一种用于制备和表征犬肠系膜动脉亚细胞膜的分析方法。
Prep Biochem. 1983;13(4):275-314. doi: 10.1080/00327488308068175.
7
Tris inhibits binding and transport of calcium in microsomal fraction isolated from rat vas deferens.三羟甲基氨基甲烷抑制从大鼠输精管分离的微粒体组分中钙的结合和转运。
Arch Int Pharmacodyn Ther. 1984 Jun;269(2):252-62.
8
Ca2+ handling properties of microsomal subfractions of rat vas deferens smooth muscle.大鼠输精管平滑肌微粒体亚组分的钙离子处理特性
Can J Physiol Pharmacol. 1984 Jan;62(1):76-9. doi: 10.1139/y84-011.
9
Interactions of saponin with microsomal membranes isolated from vascular smooth muscle.
Arch Int Pharmacodyn Ther. 1988 Jan-Feb;291:55-67.
10
Membrane fractionation of canine aortic smooth muscle: subcellular distribution of calcium transport activity.犬主动脉平滑肌的膜分级分离：钙转运活性的亚细胞分布
J Mol Cell Cardiol. 1984 Aug;16(8):747-64. doi: 10.1016/s0022-2828(84)80658-6.
引用本文的文献
1
Ca2+ pumps in smooth muscle cells.平滑肌细胞中的钙离子泵
J Muscle Res Cell Motil. 1993 Apr;14(2):141-57. doi: 10.1007/BF00115449.
2
Alloxan inhibits ligand binding to adrenoceptors of vascular smooth muscle microsomes.四氧嘧啶抑制配体与血管平滑肌微粒体肾上腺素能受体的结合。
Biochem J. 1990 Aug 15;270(1):137-40. doi: 10.1042/bj2700137.
本文引用的文献
1
Pathogenesis of pulmonary edema by alloxan.
Circ Res. 1957 Mar;5(2):180-6. doi: 10.1161/01.res.5.2.180.
2
Ca2+ uptake and distribution in alloxan-diabetic rat arterial and venous smooth muscle.钙在四氧嘧啶糖尿病大鼠动脉和静脉平滑肌中的摄取与分布。
Experientia. 1980 Nov 15;36(11):1298-9. doi: 10.1007/BF01969599.
3
Isolation of a plasma-membrane fraction from gastric smooth muscle. Comparison of the calcium uptake with that in endoplasmic reticulum.从胃平滑肌中分离出质膜部分。将其钙摄取与内质网中的钙摄取进行比较。
Biochem J. 1983 Feb 15;210(2):315-22. doi: 10.1042/bj2100315.
4
Isolation of plasma membranes from rat mesenteric veins: a comparison of their physical and biochemical properties with arterial membranes.大鼠肠系膜静脉质膜的分离：其物理和生化特性与动脉膜的比较。
Blood Vessels. 1981;18(4-5):171-86. doi: 10.1159/000158352.
5
Alloxan toxicity to the pancreatic B-cell. A new hypothesis.四氧嘧啶对胰腺β细胞的毒性作用。一种新假说。
Biochem Pharmacol. 1982 Nov 15;31(22):3527-34. doi: 10.1016/0006-2952(82)90571-8.
6
An analytical approach to the preparation and characterization of subcellular membranes from canine mesenteric arteries.一种用于制备和表征犬肠系膜动脉亚细胞膜的分析方法。
Prep Biochem. 1983;13(4):275-314. doi: 10.1080/00327488308068175.
7
Inhibition by dithioerythritol of alloxan induced efflux of Ca2+ and accompanying alterations in isolated liver mitochondria.
Biochem Int. 1984 Apr;8(4):597-602.
8
Differentiation of Ca2+ pumps linked to plasma membrane and endoplasmic reticulum in the microsomal fraction from intestinal smooth muscle.小肠平滑肌微粒体组分中与质膜和内质网相关的钙离子泵的分化
Biochim Biophys Acta. 1981 Dec 21;649(3):651-60. doi: 10.1016/0005-2736(81)90170-x.
9
Characteristics of calcium transport and binding by rat myometrium plasma membrane subfractions.
Am J Physiol. 1980 Sep;239(3):C66-74. doi: 10.1152/ajpcell.1980.239.3.C66.
10
Effects of alloxan and streptozotocin on calcium transport in isolated mouse liver mitochondria.
Cell Calcium. 1982 May;3(2):191-8. doi: 10.1016/0143-4160(82)90014-8.
Zotero 插件