通过噬菌体对肺炎克雷伯菌K60荚膜多糖进行解聚制备支链七糖

Preparation of a branched heptasaccharide by bacteriophage depolymerization of Klebsiella K60 capsular polysaccharide.

作者信息

Di Fabio J L, Dutton G G, Parolis H

出版信息

Carbohydr Res. 1984 Mar 15;126(2):261-9. doi: 10.1016/0008-6215(84)85383-5.

Abstract

Bacteriophage phi 60 possesses an endoglucosidase that depolymerizes the capsular polysaccharide of Klebsiella K60 into a heptasaccharide having two single beta-D-glucopyranosyl side-chains. This bulky oligosaccharide may be used as a probe to examine the combining sites of immunoglobulins. The 1H- and 13C-n.m.r.-spectral data of the oligosaccharide are reported.

摘要

噬菌体φ60拥有一种内切葡糖苷酶，可将克雷伯氏菌K60的荚膜多糖解聚为一种具有两个单个β-D-吡喃葡萄糖基侧链的七糖。这种大分子寡糖可用作检测免疫球蛋白结合位点的探针。本文报道了该寡糖的1H和13C核磁共振光谱数据。