Rombout J H, Stroband H W, Taverne-Thiele J J
Cell Tissue Res. 1984;236(1):207-16. doi: 10.1007/BF00216533.
The processes of proliferation, cell division and differentiation of intestinal epithelial cells have been studied during development of the fish, Barbus conchonius. On the 3rd day, nearly all cells of the presumptive gut proliferate. Once the intestinal epithelium begins to differentiate, a decreasing percentage of proliferative cells can be found. On the 7th day, when intestinal folds start to develop, the proliferative cells become restricted to the future basal parts of the folds. Ultrastructural examination of 3H-thymidine-labeled cells and mitotic cells of 6-day-old larvae shows that functional enterocytes are proliferative. The same feature is suggested for older fish. Proliferating undifferentiated "dark" cells, characterized by many free ribosomes and a few organelles, are also present in the intestinal epithelium of larval fish; they are considered to be stem cells, mainly for goblet cells. Proliferating goblet cells and enteroendocrine cells were not observed. The latter cell type is scarce and has a long turnover time. A common feature of all these dividing cells is the presence of isolated spherical to cylindrical lamellar structures which may have lost contact with the cell membrane during prophase; they probably regain this contact by fusion with the cell membrane at the end of mitosis.
在鱼类细纹无须魮发育过程中，对其肠上皮细胞的增殖、细胞分裂和分化过程进行了研究。在第3天，几乎所有推测的肠道细胞都在增殖。一旦肠上皮开始分化，增殖细胞的比例就会下降。在第7天，当肠褶开始发育时，增殖细胞局限于褶的未来基部。对6日龄幼虫的3H-胸腺嘧啶核苷标记细胞和有丝分裂细胞的超微结构检查表明，功能性肠上皮细胞具有增殖能力。年龄较大的鱼也有同样的特征。幼虫鱼类的肠上皮中也存在增殖的未分化“暗”细胞，其特征是有许多游离核糖体和少量细胞器；它们被认为是干细胞，主要是杯状细胞的干细胞。未观察到增殖的杯状细胞和肠内分泌细胞。后一种细胞类型稀少且更新时间长。所有这些分裂细胞的一个共同特征是存在孤立的球形至圆柱形板层结构，这些结构在前期可能与细胞膜失去了接触；它们可能在有丝分裂末期通过与细胞膜融合重新建立这种接触。
