Fujimoto S, Hattori T, Kimoto K, Yamashita S, Fujita S, Kawai K
Gastroenterology. 1980 Nov;79(5 Pt 1):785-91.
The origin and renewal of the gastrin cells in the pyloric glands of hamsters were studied by [3H]thymidine autoradiography combined with the immunoperoxidase identification technique. After a single injection of [3H]thymidine, the labeling index of gastrin cells was 0.3%, indicating that a few gastrin cells at the isthmus region have a self-replicating activity. Following pulse labeling after five repeated injections of [3H]thymidine at 6-hr intervals, the labeling index of the gastrin cells showed a linear increase (from 1.2% on the 1st day to 5.2% on the 4th day), and attained a plateau (5.6%) on the 5th day. These findings indicate that most gastrin cells differentiate functionally within 5 days. On the 5th day after the last of 41 injections repeated at 6-hr intervals, the labeling index of gastrin cells was 25.8%, and it decreased gradually as follows: 22.3%, 18.3%, 11.2%, 7.3%, 0.9%, and 0% on the 10th, 15th, 20th, 30th, 50th, and 100th day, respectively. From analysis of this decreasing curve, the turnover time of the gastrin cells (a half-life of the cells) was estimated to be 10-15 days. Spatially. the labeled gastrin cells were at first found in a region from the isthmus to the upper level of the glands and later in the lower level of the glands. This indicates that the gastrin cells arising from immature precursor cells at the isthmus region migrate downwards toward the bottom of the glands.
采用[3H]胸腺嘧啶核苷放射自显影技术结合免疫过氧化物酶鉴定技术，研究了仓鼠幽门腺中胃泌素细胞的起源和更新。单次注射[3H]胸腺嘧啶核苷后，胃泌素细胞的标记指数为0.3%，表明峡部区域的少数胃泌素细胞具有自我复制活性。在以6小时间隔重复注射5次[3H]胸腺嘧啶核苷进行脉冲标记后，胃泌素细胞的标记指数呈线性增加（从第1天的1.2%增加到第4天的5.2%），并在第5天达到平台期（5.6%）。这些发现表明，大多数胃泌素细胞在5天内功能分化。在以6小时间隔重复进行41次注射中的最后一次注射后的第5天，胃泌素细胞的标记指数为25.8%，随后逐渐下降，如下所示：在第10天、15天、20天、30天、50天和100天分别为22.3%、18.3%、11.2%、7.3%、0.9%和0%。通过对这条下降曲线的分析，胃泌素细胞的更新时间（细胞的半衰期）估计为10 - 15天。在空间上，最初在从峡部到腺体上部水平的区域发现标记的胃泌素细胞，随后在腺体下部水平发现。这表明起源于峡部区域未成熟前体细胞的胃泌素细胞向下迁移至腺体底部。
