Klann R C, Marchok A C
Cell Biol Int Rep. 1984 Feb;8(2):137-46. doi: 10.1016/0309-1651(84)90080-8.
We have compared the in vitro growth and viability of tumorigenic and nontumorigenic rat tracheal epithelial cell lines over a range of calcium concentrations from 0.003 to 0.85 mM. A greater dependence on calcium for proliferation was seen in the nontumorigenic line as compared to the tumorigenic line at both the colony formation level and in mass cultures. In the latter culture condition, a marked differential effect on cell survival was also demonstrated. These differences in calcium dependence were seen in media containing fetal bovine serum or low concentrations of newborn calf serum and in a serum-free medium developed for these cells. The effect was also independent of the method used for calcium removal i.e., either by chelex treatment or the inclusion of EGTA. Therefore, loss of calcium dependence may be associated with tumorigenicity in rat tracheal epithelial cells offering a selectable marker for neoplastic cells in carcinogen-exposed preneoplastic cell populations.
我们比较了致瘤性和非致瘤性大鼠气管上皮细胞系在0.003至0.85 mM范围内一系列钙浓度下的体外生长和活力。在集落形成水平和大规模培养中，与致瘤性细胞系相比，非致瘤性细胞系对钙增殖的依赖性更强。在后一种培养条件下，还证明了对细胞存活有明显的差异效应。在含有胎牛血清或低浓度新生牛血清的培养基以及为这些细胞开发的无血清培养基中，都观察到了钙依赖性的这些差异。该效应也与用于去除钙的方法无关，即通过螯合处理或加入乙二醇双四乙酸（EGTA）。因此，钙依赖性的丧失可能与大鼠气管上皮细胞的致瘤性有关，为致癌物暴露的癌前细胞群体中的肿瘤细胞提供了一个可选择的标记。
