Swierenga S H, Whitfield J F, Karasaki S
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6069-72. doi: 10.1073/pnas.75.12.6069.
The proliferative activities of three lines of "normal" epithelioid rat liver cells and six tumorigenic liver cell lines in the presence of a wide range of calcium concentrations were measured by a simple colony forming assay. The proliferative activities of the normal cells and, to a lesser extent, of the cells of a marginally tumorigenic line were directly proportional to the extracellular calcium concentration. The proliferative activities of the cells of the strongly tumorigenic lines, on the other hand, were either uninfluenced by or inversely proportional to the extracellular calcium concentration. Thus, the proliferative response to the extracellular calcium concentration is a sensitive indicator of the carcinogenic potential of liver cells.
通过一种简单的集落形成试验，测定了三种“正常”上皮样大鼠肝细胞系和六种致瘤性肝细胞系在广泛的钙浓度范围内的增殖活性。正常细胞以及在较小程度上边缘致瘤性细胞系的细胞增殖活性与细胞外钙浓度成正比。另一方面，强致瘤性细胞系的细胞增殖活性要么不受细胞外钙浓度影响，要么与之成反比。因此，对细胞外钙浓度的增殖反应是肝细胞致癌潜力的一个敏感指标。
