Maser M D, Trimble J J
J Histochem Cytochem. 1977 Apr;25(4):247-51. doi: 10.1177/25.4.67137.
Acidified 2,2-dimethoxypropane (DMP) has been used as a rapid chemical dehydrating agent en route to plastic embedding and ulthrathin sectioning for conventional electron miscroscopy (J Histochem Cytochem 23:107, 1975). We have used DMP to dehydrate biologic specimens prior to critical point drying and metal coating for scanning electron microscopy. There is no difference in either the gross architecture or the fine surface structure of mouse small intestine and trachea, rat trachea and kidney, and cultured fibroblasts, between samples dehydrated in DMP for 5 min to 30 days and those conventionally dehydrated in ethanol or acetone. DMP dehydration is advantageous in speed, economy and apparent completeness.
酸化的2,2 - 二甲氧基丙烷（DMP）已被用作一种快速化学脱水剂，用于传统电子显微镜的塑料包埋和超薄切片过程中（《组织化学与细胞化学杂志》23:107, 1975）。我们已使用DMP在临界点干燥和金属镀膜之前对生物标本进行脱水，以用于扫描电子显微镜观察。在DMP中脱水5分钟至30天的小鼠小肠和气管、大鼠气管和肾脏以及培养的成纤维细胞样本，与在乙醇或丙酮中常规脱水的样本相比，其大体结构或精细表面结构均无差异。DMP脱水在速度、经济性和明显的完整性方面具有优势。
