一种为扫描电子显微镜制备昆虫标本的新方法。

A new method of preparing insects for scanning electron microscopy.

作者信息

Bjerke J M, Freeman T P, Anderson A W

出版信息

Stain Technol. 1979 Jan;54(1):29-31. doi: 10.3109/10520297909110672.

DOI:10.3109/10520297909110672

Abstract

Acidified 2,2-dimethoxypropane (DMP), used as an alternative to regular fixation and dehydration methods for insects, was found to be the only successful means of preparing the sugarbeet root maggot larva, Tetanops myopaeformis (Röder) (Diptera:Otitidae), for the scanning electron microscope. No morphological changes were evident when DMP treated sugarbeet root maggot adults were compared to fresh (unfixed) adults and glutaraldehyde-osmium tetroxide fixed adults. The method has been used with success on several gropus of insects. Acidified CMP is quickly hydrolyzed by water in tissue to acetone and methanol. DMP is advantageous in that it penetrates water impermeable cuticles rapidly and saves several steps and time in the fixation and dehydration process.

摘要

酸化的2,2 - 二甲氧基丙烷（DMP）被用作昆虫常规固定和脱水方法的替代方法，结果发现它是制备甜菜根蛆幼虫Tetanops myopaeformis（Röder）（双翅目：实蝇科）用于扫描电子显微镜观察的唯一成功方法。将经DMP处理的甜菜根蛆成虫与新鲜（未固定）成虫以及经戊二醛 - 四氧化锇固定的成虫进行比较时，未发现明显的形态变化。该方法已成功应用于几类昆虫。酸化的CMP在组织中会迅速被水水解为丙酮和甲醇。DMP的优势在于它能迅速穿透不透水的表皮，并且在固定和脱水过程中节省了几个步骤和时间。