Takano J
Dev Biol. 1984 May;103(1):96-108. doi: 10.1016/0012-1606(84)90011-3.
A mutant hydra strain L4 produces buds at a much lower rate than the standard wild-type strain (105) when fed with brine shrimp nauplii (T. Sugiyama and T. Fujisawa, 1979b, Dev. Growth Differ. 21, 361-375). It was found that addition of a small amount of tubifex worm tissue to the normal brine shrimp diet significantly improved the budding rate of L4 but not of 105. Detailed examination and comparison of food effect on various developmental processes in L4 and 105 have provided the following observations. (1) L4 development is strongly affected by food and has significantly lower rates than 105 in all the developmental parameters examined which involve the bud initiation process. In contrast, such effects and differences are not observed in parameters not involving bud initiation. These observations suggest that L4 has a defect(s) in its bud initiating mechanisms and that the expression of this defect is somehow strongly affected by food. (2) Epithelial cells proliferate in L4 nearly as rapidly as in 105 and without food effect. Epithelial cells produced by cell division are mostly utilized to form buds in mature 105 polyps. These cells, however, are not fully utilized for this purpose in L4 polyps which bud very slowly. Instead, they appear to be somehow lost from tissue in these animals.
一种突变型水螅菌株L4，在投喂卤虫无节幼体时，出芽速率比标准野生型菌株（105）低得多（T. 杉山和藤泽T.，1979b，《发育、生长与分化》21，361 - 375）。研究发现，在正常卤虫食物中添加少量颤蚓组织，能显著提高L4的出芽率，但对105菌株则无此效果。对L4和105菌株中食物对各种发育过程影响的详细检查和比较，得出了以下观察结果。（1）L4的发育受食物的强烈影响，在所有涉及芽起始过程的发育参数方面，其速率都显著低于105。相比之下，在不涉及芽起始的参数中未观察到此类影响和差异。这些观察结果表明，L4在其芽起始机制上存在缺陷，且这种缺陷的表达在某种程度上受到食物的强烈影响。（2）L4中上皮细胞的增殖速度与105中几乎一样快，且不受食物影响。细胞分裂产生的上皮细胞在成熟的105型息肉中大多用于形成芽。然而，在出芽非常缓慢的L4型息肉中，这些细胞并未完全用于此目的。相反，它们似乎在这些动物的组织中以某种方式消失了。
