使用垂体切除的异育银鲫进行鲑鱼催乳素的生物测定。

Bioassay for salmon prolactin using hypophysectomized Fundulus heteroclitus.

作者信息

Grau E G, Prunet P, Gross T, Nishioka R S, Bern H A

出版信息

Gen Comp Endocrinol. 1984 Jan;53(1):78-85. doi: 10.1016/0016-6480(84)90226-0.

Abstract

A bioassay for salmon prolactin (PRL) is described. This assay which is based on the sodium-retaining action of PRL in the hypophysectomized killifish, Fundulus heteroclitus, has proved to be rapid, sensitive (250 pg PRL per gram of fish), and specific. The procedure has been used to characterize the biological activity of a highly purified PRL from the pituitaries of the chinook salmon, Oncorhynchus tschawytscha, and a similar PRL isolated (by acid buffer polyacrylamide disc gel electrophoresis) from pituitaries of coho salmon (O. kisutch) (MW ca. 22,000; isoelectric point greater than 9).

摘要

本文描述了一种用于鲑鱼催乳素（PRL）的生物测定方法。该测定基于PRL在切除垂体的底鳉（Fundulus heteroclitus）中的保钠作用，已证明具有快速、灵敏（每克鱼250皮克PRL）和特异性强的特点。该方法已用于表征从奇努克鲑（Oncorhynchus tschawytscha）垂体中高度纯化的PRL以及从银鲑（O. kisutch）垂体中（通过酸性缓冲液聚丙烯酰胺圆盘凝胶电泳分离）分离出的类似PRL的生物活性（分子量约22,000；等电点大于9）。