Fehér J, Toncsev H, Sréter L, Cornides A, Kiss A
Int J Tissue React. 1984;6(1):75-80.
The authors examined the effect in vivo and in vitro of Catergen (cyanidanol-3) on demonstrable lysosomal enzyme activity in the serum and granulocytes in liver diseases. Acid phosphatase, cathepsin-D and beta-glucuronidase were investigated. In the in vitro studies the direct effect of Catergen was observed by enzyme release. In chronic active hepatitis without treatment the lysosomal activity of the serum increases, the lysosomal activity of the granulocytes decreases and in vitro release increases. Under the effects of Catergen treatment the lysosomal activity of the serum decreases in comparison with initial values, approaching the lysosomal enzyme activity observed in healthy persons. Under in vitro conditions, lysosomal enzyme release from the granulocytes decreases in treated individuals and thus a higher lysosomal enzyme activity in the granulocytes is observed. Granulocytes separated from the blood of healthy persons were incubated with Catergen in isotonic and hypotonic media. In an isotonic medium the release did not change significantly. Under the effect of hypotension the release of lysosomal enzyme increased considerably. In vitro incubation with small doses of Catergen significantly inhibited the degree of release. On the basis of these data it may be supposed that Catergen has a stabilizing effect on the lysosomal membrane and is thus beneficial in the treatment of liver injuries (alcoholic, toxic, inflammatory) with lysosomal membrane alterations.
作者研究了卡特金（花青定-3）在体内和体外对肝脏疾病患者血清及粒细胞中可检测到的溶酶体酶活性的影响。研究了酸性磷酸酶、组织蛋白酶-D和β-葡萄糖醛酸酶。在体外研究中，通过酶释放观察到卡特金的直接作用。在未经治疗的慢性活动性肝炎中，血清溶酶体活性增加，粒细胞溶酶体活性降低，体外释放增加。在卡特金治疗的作用下，血清溶酶体活性与初始值相比降低，接近健康人观察到的溶酶体酶活性。在体外条件下，治疗个体中粒细胞的溶酶体酶释放减少，因此观察到粒细胞中溶酶体酶活性较高。将从健康人血液中分离出的粒细胞在等渗和低渗培养基中与卡特金一起孵育。在等渗培养基中，释放没有明显变化。在低血压的作用下，溶酶体酶的释放显著增加。用小剂量卡特金进行体外孵育可显著抑制释放程度。基于这些数据，可以推测卡特金对溶酶体膜具有稳定作用，因此对治疗伴有溶酶体膜改变的肝损伤（酒精性、中毒性、炎症性）有益。
