Handrow R R, Boehm S K, Lenz R W, Robinson J A, Ax R L
J Androl. 1984 Mar-Apr;5(2):51-63. doi: 10.1002/j.1939-4640.1984.tb00776.x.
In Vitro binding and some binding parameters of the glycosaminoglycan heparin to viable epididymal or ejaculated bull spermatozoa, ejaculated rabbit spermatozoa, and frozen-thawed rhesus monkey spermatozoa were investigated. Nonspecific binding was affected only by the concentration of 3H-heparin, whereas specific binding was saturable, reversible, and dependent on the pH, temperature, and calcium concentration of the incubation medium. Magnesium concentration dependence was observed in the presence of calcium but could not be detected in the absence of calcium. Bound 3H-heparin was displaced by several orders of magnitude greater concentrations of chondroitin sulfate. Scatchard plot analysis suggested multiple binding affinities for 3H-heparin to spermatozoa. 3H-heparin was shown to bind to sperm heads and flagella. Fluorescein-labeled heparin bound to acrosomal, postacrosomal, and flagellar membranes. It was concluded that the specific binding of heparin involved a proteinaceous component on, or intercalated with, spermatozoal membranes. Thus, glycosaminoglycans present in the female reproductive tract may contribute to sperm capacitation and enhance the likelihood of successful fertilization in mammals.
研究了糖胺聚糖肝素与活的附睾或射出的公牛精子、射出的兔精子以及冻融的恒河猴精子的体外结合及一些结合参数。非特异性结合仅受³H - 肝素浓度的影响，而特异性结合具有饱和性、可逆性，且依赖于孵育介质的pH、温度和钙浓度。在有钙存在时观察到镁浓度依赖性，但在无钙时未检测到。结合的³H - 肝素被浓度高出几个数量级的硫酸软骨素所取代。Scatchard图分析表明³H - 肝素与精子存在多种结合亲和力。³H - 肝素显示与精子头部和鞭毛结合。荧光素标记的肝素与顶体、顶体后和鞭毛膜结合。得出的结论是，肝素的特异性结合涉及精子膜上或插入精子膜的蛋白质成分。因此，雌性生殖道中存在的糖胺聚糖可能有助于精子获能并增加哺乳动物成功受精的可能性。
